High-Density Genetic Map and QTL Analysis in Cunninghamia lanceolate: Insights into Growth and Wood-Color Traits

Cunninghamia lanceolata is one of the most important tree species in China due to its significance both economy and ecology. The aims present study were construct a high-density genetic map identify quantitative trait locus (QTL) for C. lanceolata. In this study, an F1 population comprising 81 individuals was developed. Using specific length amplified fragment sequencing (SLAF-seq) technology, total 254,899 loci found be polymorphic. After linkage analysis, 2574 markers used maps. Specifically, 1632 allocated 11 groups (LGs) female map, 1038 male integrated map. consisted 4596 single-nucleotide polymorphisms (SNPs) loci, resulting average 1.79 SNP per SLAF marker. marker coverage 1665.76 cM 1436.39 1748.40 interval between two adjacent mapped 1.03 cM, 1.40 0.68 respectively. we performed mapping (logarithm odds, LOD > 2.0) detect traits interest. We identified 2, 1, 5, 3 QTLs diameter at breast height, heartwood diameter, proportion, a*, b*, L*, sapwood number associated with each QTL ranged from 1 14, explained phenotypic variances ranging 12.70% 23.60%. Furthermore, common height color while another observed L* a*. These findings suggest possible pleiotropic effects same genes on these traits. conclusion, successfully constructed maps using SLAF-seq method population. Notably, represent comprehensive densest ones available date will facilitate future chromosome assignments whole-genome sequencing. serve as valuable resource conducting fine-scale implementing marker-assisted selection lanceolata, particularly growth wood-color